| | Mikenna Vanderheyden | Strathroy - Strathroy (Strathroy ) | 4:55.39 | 4:51.83q | |
| | Kylee Raftis | Bishop Strachan School | 5:07.71 | 5:10.01q | |
| | Katherine Sheridan | Bell H.S - Nepean | 5:13.01 | 5:11.76q | |
| | Chloe Walker | Vincent Massey - Windsor (Windsor ) | 5:10.92 | 5:12.38q | |
| | Alison White | Greenwood College | 5:12.71 | 5:12.66q | |
| | Sarah Turner | Sir Oliver Mowat - West Hill | 5:10.32 | 5:14.42q | |
| | Madison Greenhough | Sir W Churchill SS - St Cather | 5:27.44 | 5:14.70q | |
| | Hannah Frazer | Sinclair - Whitby ( ) | 5:19.61 | 5:15.27q | |
| | Sadie-Jane Hickson | Lindsay CVI - Lindsay | 5:07.95 | 5:15.41q | |
| | Avery Dolderman | Centre Wellington - Fergus (Fergus ) | 5:20.63 | 5:15.87q | |
| | Anna Bout | St. John's Kilmarnock | 5:14.14 | 5:17.51q | |
| | Renelle Briggs | Sinclair - Whitby ( ) | 5:22.47 | 5:19.38q | |
| | Kate Knafelc | E. L. Crossley CSS - Fonthill | 5:29.97 | 5:19.41 | |
| | Nicole Sartor | St Maximilian Kolbe CHS Aurora | 5:22.71 | 5:21.98 | |
| | Kat Grgic | St Aloysius Gonzaga SS -Missis | 5:25.55 | 5:23.05 | |
| | Meghan Boileau | ESC Theriault - Timmins (Timmins ) | 5:37.31 | 5:28.15 | |
| | Ella Kubas | Milton District HS - Milton | 5:31.79 | 5:29.92 | |
| | Holly Hanchiruk | Huron Park - Woodstock (Woodstock ) | 5:21.03 | 5:34.76 | |
| | Marin Scotten | Sir Wilfrid Laurier SS -Orlean ( ) | 5:15.05 | 5:37.55 | |
| | Laura Carlton | Bracebridge MLSS - Bracebridge | 5:30.04 | 5:38.10 | |
| | Nathalie Marks De Chabris | Lo-Ellen Park SS - Sudbury (Sudbury ) | 5:48.87 | 5:46.57 | |
| | Corinne Schonewille | Brockville - Brockville | 5:15.70 | 5:48.14 | |
| | Kennedy Parks | Espanola HS - Espanola (Espanola ) | 6:08.12 | 6:10.03 | |
| | Sarah Urquhart | Dryden HS - Dryden (Dryden ) | 6:19.31 | 6:18.31 | |
