| | Ariana Jorgensen | Emily Carr - Vaughan (Vaughan) | 46.62 | 46.63q | |
| | Brittany Lewis | St. Clair - Sarnia (Sarnia ) | 44.72 | 46.74q | |
| | Katie Kalchman | E. L. Crossley - Fonthill (Fonthill) | 48.88 | 48.05q | |
| | Ocian Archer | Innisdale - Barrie (Barrie ) | 47.49 | 47.60q | |
| | Lindsay Wallace | Nelson - Burlington (Burlington ) | 47.52 | 48.14q | |
| | Jessica Fullerton | Bearcreek - Barrie (Barrie ) | 48.67 | 48.66q | |
| | Jordan Gray | Sinclair - Whitby (Whitby ) | 47.88 | 48.18q | |
| | Kristina Brown | Country Day School - King City (King City ) | 50.45 | 48.43q | |
| | Alannah MacLean | Lo-Ellen Park - Sudbury (Sudbury) | 49.69 | 49.07 | |
| | Nicollette Weathers | Father Redmond - Toronto (Toronto) | 49.27 | 49.31 | |
| | Elizabeth Vandenborn | Chatham-Kent - Chatham (Chatham) | 47.46 | 49.32 | |
| | Jen Barbon | Mother Teresa - London (London) | 47.75 | 49.38 | |
| | Hillary Beattie | Grimsby - Grimsby (Grimsby ) | 50.02 | 49.54 | |
| | Megan Farrow | Kingston - Kingston (Kingston ) | 50.21 | 49.75 | |
| | Alex Rudow | Bluevale - Waterloo (Waterloo ) | 47.47 | 49.93 | |
| | Beth Farnell | Kingston - Kingston (Kingston ) | 50.24 | 50.13 | |
| | Jenna Barr | Almonte - Almonte (Almonte ) | 49.87 | 50.20 | |
| | Jennifer Weir | Centennial - Welland (Welland ) | 52.99 | 50.49 | |
| | Hillary MacMillan | Renfrew - Renfrew (Renfrew ) | 49.75 | 50.58 | |
| | Caitlin Lanktree | Northern - Toronto (Toronto) | 49.91 | 51.21 | |
| | Clio Fregoli | Michael Power/St.Joseph-Etobic (Etobicoke ) | 52.05 | 51.63 | |
| | Hannah Britton-Foster | Elliot Lake - Elliot Lake (Elliot Lake) | 51.29 | 52.67 | |
| | Katarina Schwabe | Lo-Ellen Park - Sudbury (Sudbury) | 53.82 | 52.77 | |
| | Breanna Atkinson | Robert F. Hall - Caledon East (Caledon East) | 50.37 | 53.86 | |
