| | Caileigh Glenn | Sacred Heart C H S - yrsh (Newmarket ) | 2:17.76 | 2:19.78q | |
| | Rachel Islam | Innisdale S S - gbid (Barrie ) | 2:19.56 | 2:19.87q | |
| | Shannon Kennedy | Martingrove C I - twmg (Islington ) | 2:18.05 | 2:20.30q | |
| | Katie O'Hagan | Markham D H S - yrma (Markham ) | 2:19.23 | 2:19.88q | |
| | Linnea Sigfridssan | Sinclair S S - losi (Whitby ) | 2:19.81 | 2:22.46q | |
| | Leanne Fernandes | Pope John Paul I I - tdjp (Scarborough ) | 2:22.98 | 2:22.64q | |
| | Fiona Callender | Bell H S - ncbe (Nepean ) | 2:27.76 | 2:19.90q | |
| | Caroline Ehrhardt | Espanola H S - noes (Espanola) | 2:25.95 | 2:21.55q | |
| | Charlotte Gilbeault | F.J. Brennan H S - swbn (Windsor ) | 2:23.81 | 2:21.98 | |
| | Samantha Kellam | General Amherst D H S - swam (Amherstburg ) | 2:22.71 | 2:22.64 | |
| | Maddie Davidson | Northern S S - tsno (Toronto) | 2:27.92 | 2:22.84 | |
| | Leah Ditchfield | London Central S S - wolo (London ) | 2:23.28 | 2:24.20 | |
| | Kodee Williams | Birchmount Park C I - tebm (Scarborough ) | 2:26.94 | 2:25.81 | |
| | Carise Thompson | Highland S S - sohi (Dundas ) | 2:30.03 | 2:25.85 | |
| | Jade Johnson | Abbey Park SS - ghap ( Oakville) | 2:27.40 | 2:26.12 | |
| | Taylor Sills | Ernestown S S - eoet (Odessa ) | 2:32.72 | 2:28.95 | |
| | Valerie Bougie | Hawkesbury ES - eohb (Hawkesbury ) | 2:26.71 | 2:30.12 | |
| | Erika Niemi | Earl of March S S - ncem (Kanata ) | 2:29.04 | 2:32.29 | |
| | Sarah Crammer-Byng | Loyola C S S - rplo (Mississauga ) | 2:30.83 | 2:32.69 | |
| | Chantal Powers | Espanola H S - noes (Espanola) | 2:34.37 | 2:33.14 | |
| | Shelby Dunbar | West Park S S - sowp (St. Catharines) | 2:30.73 | 2:36.34 | |
| | Carly Wetzl | Sir James Dunn C & V S - nodu (Sault Ste. Marie) | 2:35.46 | 2:38.08 | |
| | Julie Caverson | Lockerby C S - nolk (Sudbury) | 2:42.27 | SCR | |
| | Jane Nickson | John Paul II S S - wopa (London ) | 2:26.76 | SCR | |
