| | Tia Semplonius | St. Benedict C S S - cwsb (Cambridge ) | 25.69 | 25.14 | 2.3 | |
| | Sarah Gariba | Colonel By S S - nccl (Gloucester ) | 26.17 | 25.76 | 1.3 | |
| | Kristy Hill | Lakefield D S S - cola (Lakefield ) | 25.99 | 26.31 | -0.2 | |
| | Flo Peters | Grand River C I - cwgr (Kitchener ) | 26.86 | 25.84 | 1.3 | |
| | Adriana Allen | Pickering H S - lopi (Ajax ) | 26.55 | 26.05 | 2.3 | |
| | Olivia Sergeant | Glendale H S - wogd (Tillsonburg) | 26.87 | 26.61 | -0.2 | |
| | Chelsea Duncan | Sir Winston Churchill SS -soch (St. Catharines) | 27.11 | 26.04 | 1.3 | |
| | Jaide Lyons | F.J. Brennan H S - swbn (Windsor ) | 27.02 | 26.14 | 2.3 | |
| | Renee Ottey | Cedarbrae C I - tece (Scarborough ) | 26.26 | 26.25 | 1.3 | |
| | Camille Wallace | Cardinal Newman H C - tdne (Scarborough ) | 26.74 | 26.50 | 2.3 | |
| | Ayesha Rumble | Pine Ridge S S - lopr (Pickering ) | 26.87 | 26.52 | 1.3 | |
| | Hannah Braithwaite | Aurora H S - yrau (Aurora ) | 26.82 | 26.67 | 2.3 | |
| | Alex Giles | Governor Simcoe S S - sogs (St. Catharines) | 26.70 | 26.75 | -0.2 | |
| | Olivia Creary | Our Lady of Mount Carmel -rpmc (Mississauga ) | 26.69 | 26.78 | 2.3 | |
| | Sandra Ristich | R.H. King Academy - teki (Scarborough ) | 27.06 | 26.79 | 2.3 | |
| | Heather Schmidt | Churchill SS - nwcl ( ) | 26.70 | 26.83 | 1.3 | |
| | Lisa Kerry | Nicholson C C - coni (Nicholson ) | 26.50 | 26.88 | -0.2 | |
| | Kaitlyn Morin | St. Mary's College S S - nosm (Sault Ste. Marie) | 26.71 | 26.91 | -0.2 | |
| | Kristen Pizycki | Denis Morris H S - sodm (St. Catharines) | 26.84 | 26.98 | -0.2 | |
| | Jenna Challenger | Crestwood S S - cocr (Peterborough) | 27.34 | 27.00 | 1.3 | |
| | Ally Mooney | Bearcreek S.S. - gbbr (Barrie ) | 26.70 | 27.03 | 1.3 | |
| | Bianca Forbes | C. W. Jefferys C I - tnje (North York ) | 27.08 | 27.25 | -0.2 | |
| | Érica Maillé | Ste. Marie E S - nesm (New Liskeard ) | 27.90 | 28.36 | -0.2 | |
