| | Christy Bray | St. Theresa's HS-Midland (Midland) | 47.08 | 47.29q | |
| | Melanie Belore | West Charleton-Dunrobin (Dunrobin) | 48.18 | 47.29q | |
| | Christie Archer | Elmvale DHS-Elmvale (Elmvale) | 47.53 | 47.79q | |
| | Lisa McCutcheon | Napanee DSS-Napanee (Napanee) | 47.65 | 48.46q | |
| | MAGGIE LYNCH | Northern CIVS-Sarnia (Sarnia) | 48.00 | 48.68q | |
| | Ashley Ternovan | St. Anne SS-Windsor (Windsor) | 47.90 | 49.18q | |
| | Samantha McConnell | O'Neill CVI-Oshawa (Oshawa) | 49.90 | 48.27q | |
| | Shannon Penfold | Korah CVS-Sault Ste. Mari (Sault Ste. Marie) | 48.40 | 48.87q | |
| | Andrea White | Nepean HS-Ottawa (Ottawa) | 49.14 | 49.26 | |
| | Stephanie Conley | Charbonnel, Msgr.,De-Will (Willowdale) | 49.78 | 49.35 | |
| | Oksana Pyzik | Lo-Ellen Park SS-Sudbury (Sudbury) | 48.80 | 49.48 | |
| | DIANA FARLOW | Northern CIVS-Sarnia (Sarnia) | 49.00 | 49.55 | |
| | Alicia Davey | Preston HS-Cambridge (Cambridge) | 48.90 | 49.68 | |
| | Anik Lambert | Theriault ES-Timmins (Timmins) | 50.36 | 50.29 | |
| | Lindsay Williamson | Grimsby DSS-Grimsby (Grimsby) | 50.28 | 50.41 | |
| | Jennifer Mason | Silverthorn CI-Etobicoke (Etobicoke) | 51.47 | 51.30 | |
| | KAYLA LEEFLANG | Perth & District CI-Perth (Perth) | 51.04 | 52.48 | |
| | Jennifer Kennedy | Barton SS- Hamilton (Hamilton) | 55.45 | 53.25 | |
| | Olivia Reed | Our Lady of Mount Carmel (Mississauga) | 52.25 | 53.82 | |
| | Paria Pishva | Francis Libermann-Scarbor (Scarborough) | 50.22 | 53.92 | |
| | Karen Mann | St. Andrews Jr. HS | 53.31 | 55.06 | |
| | Alison Darling | MacDonald-Cartier-Sudbury (Sudbury) | 55.60 | 56.05 | |
| | Laura Joudrie | Ancaster HVS-Ancaster (Ancaster) | 53.88 | 58.21 | |
| | Ellyse DeCristoforo | Cardinal Carter SS-Aurora (Aurora) | 51.24 | SCR | |
