| | Ciara Costa | St. Thomas Aquinas - Oakville (Oakville) | 44.02 | 42.29 | 10 | |
| | Aliyah Campbell | Notre Dame - Ajax (Ajax) | NT | 43.84 | 8 | |
| | Juliet Chieduch | Country Day School - King City (King City) | 48.50 | 44.42 | 6 | |
| | Sabrina Milne | Notre Dame - Toronto (Toronto) | NT | 46.78 | 5 | |
| | Kaya Nishi | Humberside - Toronto (Toronto) | NT | 47.15 | 4 | |
| | Rayna Banks | Bishop Allen Academy-Etobicoke (Etobicoke) | NT | 47.49 | 3 | |
| | Hannah Lanting | Guido deBres (Hamilton) | NT | 48.26 | 2 | |
| | Ewa Chand | Bishop Allen Academy-Etobicoke (Etobicoke) | NT | 48.67 | 1 | |
| | Chantel Cabral | James Cardinal McGuigan (Downsview) | NT | 49.15 | | |
| | Fareedah Ligali | James Cardinal McGuigan (Downsview) | NT | 49.49 | | |
| | Morgan DeKlerk | Guido deBres (Hamilton) | NT | 49.77 | | |
| | Justine Peat | Notre Dame - Ajax (Ajax) | NT | 49.83 | | |
| | Kenzie Bartels | Guido deBres (Hamilton) | NT | 49.87 | | |
| | Chaeli Cotter | Marshall McLuhan - Toronto (Toronto) | NT | 50.09 | | |
| | Marieka DaSilva | Guido deBres (Hamilton) | NT | 50.88 | | |
| | Natalie Koenig | Bishop Allen Academy-Etobicoke (Etobicoke) | NT | 51.16 | | |
| | Cara Bouwman | Guido deBres (Hamilton) | NT | 51.67 | | |
| | Valerie Volkov | Bishop Allen Academy-Etobicoke (Etobicoke) | NT | 52.19 | | |
| | Ashley McNeil | Guido deBres (Hamilton) | NT | 52.55 | | |
| | Zoe Tel | Guido deBres (Hamilton) | NT | 53.10 | | |
| | Chloe Zarzoso | Notre Dame - Toronto (Toronto) | NT | 53.39 | | |
| | Mackenzie DeJesus | Mary Ward - Scarborough (Scarborough) | NT | 53.47 | | |
| | Arianna Aburto | Father John Redmond - Toronto (Etobicoke) | NT | 53.64 | | |
| | Sarah Bernardo | Mary Ward - Scarborough (Scarborough) | NT | 53.87 | | |
| | Mariam Hossieni | C. W. Jefferys - North York (North York) | NT | 54.25 | | |
| | Amalia Lala-Laurin | Notre Dame - Toronto (Toronto) | NT | 54.38 | | |
| | Alyvia Phillips | Notre Dame - Ajax (Ajax) | 50.00 | 54.64 | | |
| | Julissa Sinclair | James Cardinal McGuigan (Downsview) | NT | 56.46 | | |
| | Beatriz Lemos | Guido deBres (Hamilton) | NT | 56.51 | | |
| | Breanna Roberts | C. W. Jefferys - North York (North York) | NT | 56.94 | | |
| | Sophia Quinn | Father John Redmond - Toronto (Etobicoke) | NT | 57.55 | | |
| | Sofia Salter | Father John Redmond - Toronto (Etobicoke) | NT | 58.00 | | |
| | Jennifer Guna | Mary Ward - Scarborough (Scarborough) | NT | 58.14 | | |
| | Kayleigh Ocampo | Wexford C.S.A.- Scarborough (Scarborough) | 55.00 | 58.76 | | |
| | Madi Perry | Humberside - Toronto (Toronto) | NT | 58.95 | | |
| | Theodore Sharifi | Winston Churchill- Scarborough (Scarborough) | NT | 59.07 | | |
| | Olivia Hill | Bishop Allen Academy-Etobicoke (Etobicoke) | NT | 1:02.27 | | |
| | Shivani Jaikaran | Notre Dame - Ajax (Ajax) | NT | 1:03.05 | | |
| | Naomi Lianne Saavedra | St. Joan of Arc - Scarborough (Scarborough) | NT | 1:04.61 | | |
| | Kelly Trac | C. W. Jefferys - North York (North York) | NT | 1:10.84 | | |
| | Norvisi Tettey | Notre Dame - Ajax (Ajax) | NT | 1:13.35 | | |
| | Zainab Azam | St. Joan of Arc - Scarborough (Scarborough) | NT | 1:26.99 | | |
| | Emma Escallon-Sotomayor | Leaside - Toronto (Toronto) | NT | DNS | | |
| | Khryssiamae Bulatao | Mary Ward - Scarborough (Scarborough) | NT | DNS | | |
