| | Taylor Campeau | Essex - Essex | WEST/SWOSSAA | 4:54.70 | 4:48.79q | |
| | Emily Bryce | SAUGEEN DISTRICT | WEST/CWOSSA | 4:48.40 | 4:50.83q | |
| | Abby Ylipahkala | Sir William Mulock - Newmarket | CENTRAL/YRAA | 4:44.60 | 4:48.98q | |
| | Sophie Coutts | Leaside - Toronto | METRO/TDCAA | 4:49.14 | 4:51.79q | |
| | Sophia Lucki | Bishop Allen Academy-Etobicoke | METRO/TDCAA | 4:49.53 | 4:49.13q | |
| | Kate Botha | St. Clement's School - Toronto ( ) | METRO/CISAA | 4:51.17 | 4:52.12q | |
| | Jayden Keeler | Innisdale - Barrie | CENTRAL/GBSSA | 4:53.64 | 4:49.96q | |
| | Elissa Frielink | Unity Christian - Barrie | CENTRAL/GBSSA | 4:52.67 | 4:52.76q | |
| | Jordyn Bartolomucci | ESC Theriault - Timmins (Timmins ) | NORTH/NEOAA | 5:08.30 | 4:50.28q | |
| | Chloe Coutts | Leaside - Toronto | METRO/TDCAA | 4:49.54 | 4:51.95q | |
| | Hallee Knelsen | East Elgin - Aylmer | WEST/WOSSAA | 4:55.24 | 4:52.90q | |
| | Hilary Bishop | Bishop P.F. Reding SS - Milton | SOUTH/GHAC | 5:07.77 | 4:55.67q | |
| | Isabelle Stevens | Ashbury College - Ottawa | EAST/NCSSAA | 5:06.65 | 5:01.05 | |
| | Rachel Love | ES Sainte-Famille - Mississaug | SOUTH/ROPSSAA | 5:11.82 | 5:01.57 | |
| | Alissa Mete | Notre Dame College - Welland | SOUTH/SOSSA | 5:07.67 | 5:02.16 | |
| | Ivy Bialowas | Glebe Collegiate - Ottawa ( ) | EAST/NCSSAA | 5:05.65 | 5:04.39 | |
| | Tessa Jeffery | E. L. Crossley CSS - Fonthill | SOUTH/SOSSA | 5:03.17 | 5:04.55 | |
| | Caitlin McMann | Longfields Davidson Heights - | EAST/NCSSAA | 5:06.36 | 5:06.83 | |
| | Ana Morariu | Sacred Heart - Walkerton | WEST/CWOSSA | 4:57.34 | 5:07.20 | |
| | Kalila Bachiu | Lo-Ellen Park SS - Sudbury (Sudbury ) | NORTH/NOSSA | 5:17.64 | 5:07.45 | |
| | Maya Black | Henry Street - Whitby | CENTRAL/LOSSA | 5:06.80 | 5:08.73 | |
| | Laura Campbell | St Joseph-Scollard Hall -N Bay (North Bay ) | NORTH/NOSSA | 5:17.87 | 5:12.38 | |
| | Rachel Chayer | I.E. Weldon - Lindsay | EAST/COSSA | 5:06.84 | 5:12.47 | |
| | Grace Wilson | St Patrick HS - Thunder Bay (Thunder Bay ) | NORTH/NWOSSAA | 5:27.23 | 5:30.79 | |
