| | Jocelyn Chau | De La Salle College - Toronto | METRO/CISAA | 4:21.12 | 4:38.01q | |
| | Sam Ford | Univ of Toronto Schools - Toro | METRO/TDCAA | 4:31.69 | 4:40.01q | |
| | Cameron Ormond | Aurora High School | CENTRAL/YRAA | 4:33.74 | 4:38.13q | |
| | Haley Walker | Havergal College - Toronto | METRO/CISAA | 4:31.23 | 4:40.21q | |
| | Lilly Tuck | Port Perry - Port Perry | CENTRAL/LOSSA | 4:36.53 | 4:38.18q | |
| | Kansas MacKay | Pickering - Ajax | CENTRAL/LOSSA | 4:37.03 | 4:40.52q | |
| | Parker Hopkins | Northern - Toronto | METRO/TDCAA | 4:40.00 | 4:38.22q | |
| | Hannah Russell | Port Perry - Port Perry | CENTRAL/LOSSA | 4:37.87 | 4:41.37q | |
| | Jessica Kellar | Our Lady Mt Carmel SS -Mississ | SOUTH/ROPSSAA | 4:52.39 | 4:38.27q | |
| | Lily Meek | Thousand Islands SS | EAST/EOSSAA | 4:38.82 | 4:38.56q | |
| | Nicole Larue | St. Joseph's - Windsor | WEST/SWOSSAA | 4:41.24 | 4:43.78q | |
| | Rebecca Dekay | Sir Frederick Banting - London | WEST/WOSSAA | 4:40.94 | 4:44.64q | |
| | Hannah Tait | St. Anne's - Clinton | WEST/WOSSAA | 4:41.72 | 4:47.61 | |
| | Eirlys Rauliuk-Dunn | Deep River | EAST | 4:46.01 | 4:47.81 | |
| | Nina Whitford | Glebe Collegiate - Ottawa ( ) | EAST/NCSSAA | 4:44.23 | 4:51.54 | |
| | Maxine Dupuis | ESC Theriault - Timmins (Timmins ) | NORTH/NEOAA | 5:03.77 | 4:52.28 | |
| | Jessie Fennell | E. L. Crossley CSS - Fonthill | SOUTH/SOSSA | 5:13.22 | 4:56.02 | |
| | Kayleigh Bush | A. N. Myer SS - Niagara Falls | SOUTH/SOSSA | 5:10.02 | 4:57.37 | |
| | Amy Wickstrom | Dryden HS - Dryden (Dryden ) | NORTH/NWOSSAA | 5:07.21 | 5:08.22 | |
| | Hillary Clark | Elliot Lake SS - Elliot Lake (Elliot Lake ) | NORTH/NOSSA | 5:08.86 | 5:08.49 | |
| | Tessa Ladhani | Guelph - Guelph | WEST/CWOSSA | 4:43.51 | 5:08.54 | |
| | Sydney Tarini | Lo-Ellen Park SS - Sudbury (Sudbury ) | NORTH/NOSSA | 5:13.34 | 5:10.33 | |
| | Andrea Dodig | Humberview SS - Bolton ( ) | SOUTH/ROPSSAA | 5:26.66 | 5:21.59 | |
| | Keegan Gaunt | Merivale HS - Ottawa | EAST/NCSSAA | 4:50.05 | DNS | |
